The solid state fermentation is most commonly carried out for the production of fermented food products such as bread, fermented fish, meat, yogurt, cheese and pickles. Ssf is the preferred method for tannase production because of its lower cost, lower water consumption, easier operation, and higher enzyme activity than slf 16, 17, 18. Lonsane, in advances in applied microbiology, 1997. Industrially the production of tannase is usually performed via submerged fermentation sf. Tannase is an important enzyme which finds commercial applications in food industry to reduce the level of tannins in fruit juices, preparation of instantaneous tea and production of gallic acid. In 96th hours all the organisms produced more tannase. Tannase production by solidstate fermentation ssf is more. The effect of initial water content was studied and maximum enzyme production was obtained when 60 ml of water was added to 100. To compare the yield of tannase by pure and coculture, to enhance the yield of tannase by using carbon glucose.
Solid state fermentation offers a number of economic advantages over conventional submerged fermentation for enzyme production mudgett, 1986. Pdf production of tannase under solidstate fermentation using. Production of tannase from aspergillus niger under solid state fermentation zainab saadi mahdi, shatha salman altahan department of biology, college of science, university of baghdad, baghdad, iraq abstract thirteen a. Smf is usually implemented in case of bacterial enzyme production, due to the requirement.
Tannase production in solid state fermentation using groundnut shell tannase production by a. Ucp1284 using cashew bagasse under solid state fermentation find. In ssf with jamun leaves, the maximum production of tannase was found to be at 30 degrees c after 96 h of incubation. Castor bean residue, a toxic residue and byproduct of brazilian biodiesel production, was inoculated with a strain of p. Solid state fermentation for tannase production by fungal. Research on conditions of solidstate fermentation and. Research article purification and characterization of. Extra cellular production of tannase by aspergillus foetidus, rhizopus oryzae pure culture and co culture under solidstate fermentation using tamarind seed powder was evaluated. The optimum ph of the moistening agent was found to be 5. The substrate used for the production of tannase was tamarind seed powder a similar work was done by sabu et al. Production of tannase from aspergillus ruber under solid state fermentation using jamun syzygium cumini leaves. The fungal strain was able to grow on cab without any supplementation but a.
Substantial quantities of the enzyme were synthesized throughout the operating periods and imperfect steady. Mani 3 1 post graduate and research department of microbiology,dr. Production of tannase under solidstate fermentation and. Tannase production by aspergillus niger aa20 was studied in submerged smf and solid state ssf fermentation systems with different tannic acid and glucose concentrations. Enzyme puri cation was achieved by a combination of concentration by rotary vacuum evaporator followed by ammonium sulphate precipitationand chromatographicresolutionusing deaecellulose column. Various low cost tannin rich residues such as coffee husk, tamarind seed powder, tea leaves and triphala powder were studied in semi solid state fermentation process. Jamun leaves were found to be the best substrate for enzyme production under solid state fermentation ssf. An autochthonous tannase yielding yeast strain pichia kudriavzevii gu939629, isolated from the gut of an indian major carp mrigal, cirrhinus cirrhosus, has been used for extracellular tannase production and subsequent processing of two plant feedstuffs, groundnut oil cake goc and pistia leaves pl, under solid state fermentation ssf. Arts and science college, coimbatore 641 035 india.
The fungal culture produced high yields of extracellular tannase under solid state fermentation ssf using different agro forest residues such as amla leaves phyllanthus emblica, ber leaves zyzyphus mauritiana. Promising substrates to increase the production of tannase. Tannase production by penicillium purpurogenum paf6 in. Tannase production by aspergillus fumigatus ma under solidstate. Tannase production under solid state fermentation was investigated using isolated aspergillus oryzae. Tap water was found to be the best moistening agent, with ph 5. Improved production of tannase by klebsiella pneumoniae. Solid state fermentation ssf was evaluated to produce tannase from aspergillus niger. Fermentation is the primary technique for the production of various enzymes. The metabolic processes of microorganisms are influenced by changes in parameters like temperature, ph, incubation time, humidity etc. Solid state fermentation technology ssf easy biology class. Pdf production of tannase under solidstate fermentation. The ability of aspergillus oryzaefor the production of tannase by solid state fermentation was investigated using cashew apple bagasse cab as substrate.
Tannase production by penicillium purpurogenum paf6 in solid state fermentation of tanninrich plant residues following ovat and rsm. Pdf tannase enzyme production was studied under solid state fermentation using different tannin rich substrates like ber leaves zyyphus. Both fungi and bacteria yield an invaluable array of enzymes when fermented on appropriate substrates. The production medium is often simple, using agroindustrial byproducts like wheat bran, rice bran, or wheat. The best favourable conditions for enzyme production include initial ph 5 with 4 days of incubation period at 40c and 72% humidity, and 10 g wheat bran soaked in 2. Through the selected fungi, a complex study was achieved about the influence of the. Improved production of tannase by klebsiella pneumoniae using indian gooseberry leaves under submerged fermentation using taguchi approach mukesh kumar1,2, amrinder singh1,3, vikas beniwal 4 and raj kumar salar1 abstract tannase tannin acyl hydrolase e. The microbial fermentation increases the nutrient content and flavor of food products. The production medium is often simple, using agroindustrial byproducts like wheat bran, rice bran, or wheat straw as the substrate mitchell. Pdf production of tannase from aspergillus ruber under. Perspectives of solid state fermentation for production of.
Tannase production by aspergillus niger aa20 was studied in submerged smf and solidstate ssf fermentation systems with different tannic acid and. Initially maize, rice, wheat, barley, wheat bran were compared as carrier. An attempt has been made to optimize the production of enzyme tannase by solid state fermentation ssf using the organism rhizopus oryzae. Tannase production by solid state fermentation of cashew. The experiments involved in the process and conditions of solid state fermentation and factors influencing characters of tannase. However, tannins present in the guava juice form tanninprotein complexes that affect the utilization of vitamins and minerals and inhibit digestive.
The industrial production of tannase can be performed through solid state fermentation ssf by the use of tannin rich fruit residues, in order to minimize production costs. Production of tannase by aspergillus niger aa20 in submerged and solid state fermentation. Tannase is an industrially important enzyme produced by a large number of microorganisms. Production of tannase under solid state fermentation tea stalk powder 5 g was placed in 250ml erlenmeyer flasks and moistened with a mineral salt solution 0. Solid state fermentation ssf solid state fermentation ssf is a fermentation method used by several industries like the pharmaceuticals, food, textile etc. Production of tannase by various fungal cultures in solid. The highest yield of tannase was obtained at 96 hour 4th day incubation period, at 300c temperature and ph 5. Solid state fermentation involves nonaseptic conditions with the use. Production of tannase by aspergillus species ucp1284 was studied using solid state fermentation and cashew bagasse as substrate. Production of tannase by aspergillus niger aa20 in submerged and. Both solid state and submerged fermentation are used for enzyme production. The ssf is alternative to submerged fermentation for production. Demain al, solomon na eds manual of industrial microbiology and biotechnology. Optimization of culture conditions for tannase production.
A tannase yielding fungal culture identified as aspergillus fumigatus ma was isolated from the effluent collected from a local small scale tannery. Production of tannase from aspergillus niger under solid. The tannase enzyme production was compared under submerged and solid state fermentation conditions using aspergillus niger. Production of tannase from aspergillus ruber under solidstate. The ssf is alternative to submerged fermentation for production of value added products like. Optimization of tannase production and improvement of. Optimization of tannase production by aspergillus niger in solid. Amount of 35 strains of fungi species isolated from soil of caatinga were used for qualitative selection of strains with potential for production of tannase. Guava juice is known to be rich in antioxidant activity due a high level of vitamins a and c. This is the first report on production of tannase by aspergillus. In the present study 31 species of penicillium isolated from caatinga and atlantic forest were tested quantitatively for the production of tannase. Enzyme production was studied under solid state fermentation using different tannin.
Experimental design results 2 3 for tannase production at 96 h of ssf. Production of mevastatin by solidstate fermentation using. Production, characterization of tannase from penicillium. It is defined as the growth of microbes without freeflowing aqueous phase. Production of tannase by citrobacter freundii under solid state fermentation and its application in fruit juices debittering s. A promising method for production and optimization of tannase from bacillus gottheilii m2s2, abstract tannase is an important enzyme which finds commercial applications in food industry to reduce the level of tannins in fruit juices, preparation of instantaneous tea and production of gallic acid. Solidstate fermentation ssf is defined as the growth of microbes without free flowing aqueous phase. Mucor circinelloides, tannase, 18s rrna gene sequencing, sequential statistical strategy, solid state fermentation. Crude tannase enzyme activity at various time intervals for each of the fungus was analyzed by spectrophotometric method.
Production of tannase by aspergillus niger aa20 in. Among all agroindustrial waste material evaluated, cashew husk supported maximum tannase production. Tannase finds application in many industrial sectors which includes pharmaceutical, food, chemical and beverages industry. Production of tannase by citrobacter freundii under solid. Study is on the solid state fermentation of tannase with the gallnut by aspergillus niger b0201. After cooling, the samples were inoculated with some.
Tannase production methods include solidstate fermentation ssf 11,12, and submerged liquid fermentation slf 11, 14, 15. Solidstate fermentation an overview sciencedirect topics. Statistical optimization for tannase production by. Alternatively, to minimize production costs, there is the solid state fermentation ssf. Novel optimization strategy for tannase production through. A modified solid state fermentation was used to produce mevastatin by penicillium citrinum ncim 768 using wheat bran as carrier in glycerol and urea based medium as moistening agents. The results indicated that the unpasteurized method before fermentation was in favor of the higher enzymic activity than the sterilized method. Novel optimization strategy for tannase production through a. Production of enzyme tannase by solid state fermentation. Production of tannase under solid state fermentation tea stalk powder 5g wasplaced in 250mlerlenmeyer.
Production of tannase under solidstate fermentation using amla and jamun leaves article pdf available in annals of biology 251. Tannase production by solid state fermentation of cashew apple. Tannase production by aspergillus fumigatus ma under solid. The ability of aspergillus oryzae for the production of tannase by solid state fermentation was investigated using cashew apple bagasse cab as substrate. Three types of tannic acid media were used for submerged fermentation. Production of tannase from aspergillus ruber under solid.
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